Upgrading the use of the gene scissor –an innovation of importance for a future resource-efficient, circular, sustainable food production
Potato is one of the world’s most important food crops, but develop new varieties through conventional breeding is a slow process that often takes more than 15 years. Modern gene-editing tools such as CRISPR enable faster and more precise modifications of plant genomes. However, introducing multiple genes at once, especially large DNA sequences, remains a technical challenge. This project aims to develop a new method that allows the insertion of multiple genes into potato in a single step.
The goal is to generate cis-genic plants that are classified as “conventional-like” under the proposed EU legislation for new genomic techniques. This would enable rapid stacking of genes required for important traits, such as resistance to late blight, a devastating potato disease currently controlled with large amounts of pesticides. To validate the approach, we will work with potato cells (protoplasts), allowing rapid assessment of gene insertion efficiency and functionality without the need to regenerate whole plants.
Advanced quantitative PCR and DNA sequencing will be used to analyse outcomes and identify optimal strategies. If successful, this method will significantly accelerate the development of new potato varieties. In the long term, it will reduce not only the need for pesticides but also fertilizers, improve crop resilience to climate change, and enhance nutritional value. Overall, the project supports more sustainable agriculture and the transition to a circular bioeconomy.
